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Abstract Improvements in analytical procedures in parallel with intercalibration of⁴⁰Ar/³⁹Ar and U–Pb methods and laboratories, spurred since 2003 by the EarthTime geochronology community initiative, have led to ±2σuncertainties of the order of 50–100 ka, or better, for Cretaceous ash beds. Assembled here are 57⁴⁰Ar/³⁹Ar ages and 17²³⁸U–²⁰⁶Pb ages of volcanic ash beds in strata of the Western Interior Basin of North America determined during the last 15 years since these improvements have been made. These age determinations span from 108 Ma in the middle Albian to 66 Ma in the latest Maastrichtian. Five of the⁴⁰Ar/³⁹Ar ages from Campanian and Maastrichtian strata are newly reported here, whereas the remainder are from the literature. Building on the pioneering work of John Obradovich and Bill Cobban, where possible these age determinations are tied to ammonite and inoceramid biostratigraphy. For most ash beds, the temporal uncertainties, unlike earlier timescales for the Western Interior Basin, are much shorter than the duration of fossil biozones. Proposed ages for stage boundaries based on this review of the radioisotopic ages include: Maastrichtian–Danian, 66.02 ± 0.08 Ma; Campanian–Maastrichtian, 72.20 ± 0.20 Ma; Santonian–Campanian, 84.19 ± 0.38 Ma; Coniacian–Santonian, 86.49 ± 0.44 Ma; Turonian–Coniacian, 89.75 ± 0.38 Ma; Cenomanian–Turonian, 93.95 ± 0.05 Ma; Albian–Cenomanian, 100.00 ± 0.40 Ma. Six bentonites that occur within theVascoceras diartianum, Neocardiocerus juddi, Prionocylus macombi, Scaphites preventricosus, Scaphites depressusandDesmoscaphites bassleriammonite zones, dated using both⁴⁰Ar/³⁹Ar and U–Pb methods, yield ages in agreement to within 150 ka and form the backbone of the Western Interior Basin timescale. In parallel, improvements in the taxonomy of ammonites, inoceramids and foraminifera, and recent field work, are better establishing the biostratigraphic framework for these age determinations. Each of these efforts contributes to the progressive refinement of the chronostratigraphic framework of the Western Interior Basin, and enhances its utility for global correlation.